Marie Rosulkova (December 17, 1901 Pilsen - May 15, 1993 Prague) was a Czech actress. She has been playing in the theater since 19 to 91 years. As a bank clerk, she was privately trained by Marie Laudová-Hořicová.

Since 1921 the theater has been professionally played in Pilsen, České Budějovice and in the Zemské divadlo (1931-1933) in Brno. From the beginning, she played rather roles of ladies and experienced women than young naiveks (Frank Wedekind: Lulu, Ceske Budejovice, 1924, roles and rolls in the head of the station, The Snack Shop and many others).