Ann Rutherford was born in Vancouver, British Columbia, Canada. The daughter of a former Metropolitan Opera singer, John Rutherford, and her actress mother, Lillian Mansfield, Ann was destined for show business. Not long after her birth, her family moved to California, where she made her stage debut in 1925.

Ann appeared in many plays and on radio for the next nine years before making her first screen appearance in Waterfront Lady (1935). Ann's talent was readily apparent, and she was signed to three films in 1935: Waterfront Lady (1935), Melody Trail (1935), and The Fighting Marines (1935). By now, she was a leading lady in the fabled Westerns with two legends, John Wayne and Gene Autry.